Computes normalized cycle threshold (Ct) values (delta Ct) from raw quantitative polymerase chain reaction (qPCR) Ct values and conducts test of significance using t.test(). Plots expression values based from log2(2^(-1*delta delta Ct)) across groups per gene of interest. Methods for calculation of delta delta Ct and relative expression (2^(-1*delta delta Ct)) values are described in: Livak & Schmittgen, (2001) <doi:10.1006/meth.2001.1262>.
| Version: | 0.1.0 |
| Imports: | dplyr (≥ 1.0.0), rstatix (≥ 0.7.2), ggpubr (≥ 0.5.0), ggplot2 (≥ 3.4.0), magrittr (≥ 2.0.3) |
| Suggests: | knitr, rmarkdown |
| Published: | 2023-02-23 |
| DOI: | 10.32614/CRAN.package.qPCRhelper |
| Author: | Wilson Jr. Aala |
| Maintainer: | Wilson Jr. Aala <s98107020 at gs.ncku.edu.tw> |
| License: | GPL-3 |
| NeedsCompilation: | no |
| Materials: | NEWS |
| CRAN checks: | qPCRhelper results |
| Reference manual: | qPCRhelper.html , qPCRhelper.pdf |
| Vignettes: |
qPCRhelper (source, R code) |
| Package source: | qPCRhelper_0.1.0.tar.gz |
| Windows binaries: | r-devel: qPCRhelper_0.1.0.zip, r-release: qPCRhelper_0.1.0.zip, r-oldrel: qPCRhelper_0.1.0.zip |
| macOS binaries: | r-release (arm64): qPCRhelper_0.1.0.tgz, r-oldrel (arm64): qPCRhelper_0.1.0.tgz, r-release (x86_64): qPCRhelper_0.1.0.tgz, r-oldrel (x86_64): qPCRhelper_0.1.0.tgz |
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