--- title: "Modeling" output: rmarkdown::html_vignette vignette: > %\VignetteIndexEntry{Modeling} %\VignetteEngine{knitr::rmarkdown} %\VignetteEncoding{UTF-8} --- ```{r, include = FALSE} knitr::opts_chunk$set( collapse = TRUE, comment = "#>" ) ``` ## Modeling plant emergence and canopy growth using UAV data This vignette demonstrates piecewise regression using canopy data derived from UAV imagery to estimate two key parameters: * t1: days to plant emergence. * t2: days to reach maximum canopy. The data are from the University of Wisconsin-Madison potato breeding program, specifically for a partially replicated experiment. The UAV images were collected in 2020 and processed in 2024. ## Loading libraries ```{r, warning=FALSE, message=FALSE } library(flexFitR) library(dplyr) library(kableExtra) library(ggpubr) library(purrr) ``` ## 1. Exploring data We begin with the explorer function, which provides basic statistical summaries and descriptive statistics, as well as visualizations to help understand the temporal evolution of each plot. ```{r} data(dt_potato) explorer <- explorer(dt_potato, x = DAP, y = Canopy, id = Plot) ``` ```{r} names(explorer) ``` ```{r, fig.width= 8, fig.height=4, fig.alt="plot corr"} p1 <- plot(explorer, type = "evolution", return_gg = TRUE, add_avg = TRUE) p2 <- plot(explorer, type = "x_by_var", return_gg = TRUE) ggarrange(p1, p2) ``` To see more about the type of plots visit `plot.explorer()`. ```{r, echo=FALSE} kable(mutate_if(explorer$summ_vars, is.numeric, round, 2)) ``` ## 2. Regression Function Once the data have been explored, we define the expectation function. In this case, it is a piece-wise regression function with three parameters: t1, t2, and k. The function can be expressed mathematically as follows: `fn_linear_sat()` \begin{equation} f(t; t_1, t_2, k) = \begin{cases} 0 & \text{if } t < t_1 \\ \dfrac{k}{t_2 - t_1} \cdot (t - t_1) & \text{if } t_1 \leq t \leq t_2 \\ k & \text{if } t > t_2 \end{cases} \end{equation} ```{r, echo = FALSE, fig.width= 8, fig.alt="plot fn"} plot_fn( fn = "fn_linear_sat", params = c(t1 = 40, t2 = 61.8, k = 100), interval = c(0, 108), color = "black", base_size = 15 ) ``` ## 3. Fitting Models To fit the model, we use the modeler function. Here: * x specifies the days after planting (DAP), * y is the canopy variable to be modeled, * grp allows us to perform group analysis, e.g., on multiple plots. In this example, we have 196 plots but will only fit the model for plots 166 and 40 as a subset. We define the piecewise function `fn_linear_sat` and set initial values for the parameters. ```{r, warning=FALSE, message=FALSE } mod_1 <- dt_potato |> modeler( x = DAP, y = Canopy, grp = Plot, fn = "fn_linear_sat", parameters = c(t1 = 45, t2 = 80, k = 0.9), subset = c(166, 40) ) mod_1 ``` After fitting, we can inspect the model summary and visualize the fit using the plot function: ```{r, fig.width= 8, fig.height=4, fig.alt="plot fit"} plot(mod_1, id = c(166, 40)) kable(mod_1$param) ``` ## 3.1. Extracting model coefficients and uncertainty measures Once the model is fitted, we can extract key statistical information, such as coefficients, standard errors, confidence intervals, and the variance-covariance matrix for each group (plot). These metrics allow us to draw conclusions about the parameter estimates and assess the uncertainty around them. The functions `coef`, `confint`, and `vcov` are used as follows: * **coef**: Extracts the estimated coefficients for each group. * **confint**: Provides the confidence intervals for the parameter estimates. * **vcov**: Returns the variance-covariance matrix, which can be used to understand the relationships between the estimates and their variability. ```{r} coef(mod_1) ``` ```{r} confint(mod_1) ``` ```{r} vcov(mod_1) ``` ## 4. Providing different initial values The initial fit may not always be optimal, so we can adjust the initial parameter values for each plot and even fix certain parameters to improve the model. ```{r} initials <- data.frame( uid = c(166, 40), t1 = c(70, 60), t2 = c(40, 80), k = c(100, 100) ) ``` ```{r} kable(initials) ``` ```{r} mod_2 <- dt_potato |> modeler( x = DAP, y = Canopy, grp = Plot, fn = "fn_linear_sat", parameters = initials, subset = c(166, 40) ) ``` ```{r, fig.width= 8, fig.height=4, fig.alt="plot fit 2"} plot(mod_2, id = c(166, 40)) kable(mod_2$param) ``` It's important to note that providing poor initial guesses for the parameters can lead to inaccurate or unreliable model fits. For example, if we mistakenly assign t1 (the day of plant emergence) a value greater than t2 (the day of maximum canopy), the model fit can fail or produce nonsensical results. ## 5. Fixing some parameters of the model In certain cases, we may want to fix specific parameters either because they are known or because we prefer the model to leave these parameters unchanged. For example, we can fix the parameter `k`, which represents the maximum canopy value, as follows: ```{r} fixed_params <- list(k = "max(y)") ``` ```{r} mod_3 <- dt_potato |> modeler( x = DAP, y = Canopy, grp = Plot, fn = "fn_linear_sat", parameters = c(t1 = 45, t2 = 80, k = 0.9), fixed_params = fixed_params, subset = c(166, 40) ) ``` ```{r, fig.width= 8, fig.height=4, fig.alt="plot fit 3"} plot(mod_3, id = c(166, 40)) kable(mod_3$param) ``` By fixing k to 100, we are telling the model that the maximum canopy for these plots is fixed at 100%. This allows the model to focus on estimating the other parameters, t1 and t2, potentially improving the accuracy of their estimates by reducing the complexity of the model. ## 6. Comparing estimations ```{r} rbind.data.frame( mutate(mod_1$param, model = "1", .before = uid), mutate(mod_2$param, model = "2", .before = uid), mutate(mod_3$param, model = "3", .before = uid) ) |> filter(uid %in% 166) |> kable() ``` After fitting multiple models with different initial values, fixed parameters, and canopy adjustments, we can compare the resulting coefficients and sum of square errors (`sse`) to evaluate the impact of these changes. ```{r} rbind.data.frame( mutate(AIC(mod_1), model = "1", .before = uid), mutate(AIC(mod_2), model = "2", .before = uid), mutate(AIC(mod_3), model = "3", .before = uid) ) |> filter(uid %in% 166) |> kable() ``` ## 7. Making predictions Once the model is fitted and validated as the best representation of our data, we can proceed to make predictions. The `predict.modeler()` function provides a range of flexible prediction options, allowing users to perform point predictions, calculate the area under the curve (AUC), compute first or second derivatives, and even evaluate custom functions of the parameters. Below are some examples demonstrating these capabilities: ```{r} # Point Prediction predict(mod_1, x = 45, type = "point", id = 166) |> kable() # AUC Prediction predict(mod_1, x = c(0, 108), type = "auc", id = 166) |> kable() # Function of the parameters predict(mod_1, formula = ~ t2 - t1, id = 166) |> kable() ``` In each example, the `predict.modeler()` function tailors the predictions to the user’s needs, whether it's estimating a single value, integrating across a range, or calculating a parameter-based expression. ## 8. Modelling all plots using parallel processing Finally, we can apply this method to all 196 plots, leveraging parallel processing to speed up the computation. To do this, we specify `parallel = TRUE` in the options argument, and set the number of cores using the function `parallel::detectCores()`, which automatically detects the available cores. ```{r, eval= FALSE} mod <- dt_potato |> modeler( x = DAP, y = Canopy, grp = Plot, fn = "fn_linear_sat", parameters = c(t1 = 45, t2 = 80, k = 0.9), fixed_params = list(k = "max(y)"), options = list(progress = TRUE, parallel = TRUE, workers = 5) ) ```